质粒名称: | pAG001 His6-TwinStrep-SUMO-AapCas12b |
---|---|
出品公司: | Addgene |
目录编号: | 153162 |
存储实验室: | Omar Abudayyeh, Jonathan Gootenberg |
载体骨架基本信息 |
|
载体名称: | SUMO expression backbone |
载体出品公司: | Clontech |
完整质粒大小: | 9473 bp |
载体类型: | 细菌表达 |
载体抗性: | 氨苄霉素 |
生长温度: | 37 ℃ |
宿主菌: | DH5a |
拷贝数: | 未知 |
插入基因信息 |
|
插入基因名称: | SUMO expression backbone |
文献引用方法 |
|
材料和方法部分: | pAG001 His6-TwinStrep-SUMO-AapCas12b was a gift from Omar Abudayyeh & Jonathan Gootenberg (Addgene plasmid # 153162 ; http://n2t.net/addgene:153162 ; RRID:Addgene_153162) |
参考文献部分: | Point-of-care testing for COVID-19 using SHERLOCK diagnostics. Joung J, Ladha A, Saito M, Segel M, Bruneau R, Huang MW, Kim NG, Yu X, Li J, Walker BD, Greninger AL, Jerome KR, Gootenberg JS, Abudayyeh OO, Zhang F. medRxiv. 2020 May 8. doi: 10.1101/2020.05.04.20091231. |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAG001 His6-TwinStrep-SUMO-AapCas12b 9473 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Bacterial expression plasmid for AapCas12b. ACCESSION . VERSION . KEYWORDS pAG001 His6-TwinStrep-SUMO-AapCas12b SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9473) AUTHORS Joung J, Ladha A, Saito M, Segel M, Bruneau R, Huang MW, Kim NG, Yu X, Li J, Walker BD, Greninger AL, Jerome KR, Gootenberg JS, Abudayyeh OO, Zhang F TITLE Point-of-care testing for COVID-19 using SHERLOCK diagnostics. JOURNAL medRxiv. 2020 May 8. doi: 10.1101/2020.05.04.20091231. PUBMED 32511521 REFERENCE 2 (bases 1 to 9473) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.biofeng.com FEATURES Location/Qualifiers source 1..9473 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 1..20 /label=T7 /note="T7 promoter, forward primer" promoter 1..19 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 20..44 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 59..81 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 100..117 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 127..144 /codon_start=1 /product="thrombin recognition and cleavage site" /label=thrombin site /translation="LVPRGS" CDS 157..240 /codon_start=1 /product="two Strep-Tag(R)II moieties connected by a linker for enhanced binding to Strep-Tactin(R), an engineered form of streptavidin (Schmidt et al., 2013)" /label=Twin-Strep-tag /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK" CDS 241..531 /codon_start=1 /gene="S. cerevisiae SMT3 (truncated)" /product="cleavable ubiquitin-like protein tag" /label=SUMO /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIG" primer_bind complement(3974..3992) /label=T7 Term /note="T7 terminator, reverse primer" terminator 3988..4035 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" promoter complement(4210..4238) /label=tet promoter /note="E. coli promoter for tetracycline efflux protein gene" primer_bind complement(4265..4283) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4351..4455 /gene="bla" /label=AmpR promoter CDS 4456..5316 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4674..4693) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5487..6075 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5976..5995 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6229..6246 /label=L4440 /note="L4440 vector, forward primer" misc_feature 6261..6400 /label=bom /note="basis of mobility region from pBR322" primer_bind complement(6326..6345) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 6486..6508 /label=pGEX 3' /note="pGEX vectors, reverse primer" CDS complement(6502..6693) /codon_start=1 /gene="rop" /product="Rop protein, which maintains plasmids at low copy number" /label=rop /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL" CDS complement(8005..9087) /codon_start=1 /gene="lacI" /product="lac repressor" /label=lacI /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" primer_bind 9049..9068 /label=LacI-R /note="LacI, reverse primer" promoter complement(9088..9165) /gene="lacI" /label=lacI promoter primer_bind 9371..9390 /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" ORIGIN 1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt 61 tgtttaactt taagaaggag atataccatg ggcagcagcc atcatcatca tcatcacagc 121 agcggcctgg tgccgcgcgg cagccatatg gctagctgga gccatccgca gtttgaaaaa 181 ggtggtggta gcggtggtgg ttcaggtggt agtgcatggt cacaccctca gtttgagaaa 241 atgtcggact cagaagtcaa tcaagaagct aagccagagg tcaagccaga agtcaagcct 301 gagactcaca tcaatttaaa ggtgtccgat ggatcttcag agatcttctt caagatcaaa 361 aagaccactc ctttaagaag gctgatggaa gcgttcgcta aaagacaggg taaggaaatg 421 gactccttaa gattcttgta cgacggtatt agaatccaag ctgatcagac ccctgaagat 481 ttggacatgg aggataacga tattattgag gctcacagag aacagattgg tggatccatg 541 gccgtaaaat ctatgaaagt taagttgcgt ctggacaaca tgccagaaat tcgcgctggc 601 ctttggaaac tgcacaccga ggtcaatgca ggcgttcgtt attataccga gtggttaagt 661 ttattgcgtc aggaaaattt ataccgtcgc agtccaaacg gggacgggga acaggagtgt 721 tacaagacag ctgaggagtg caaggcagag ttgcttgagc gcttgcgcgc acgccaagtt 781 gagaatggac actgcggtcc ggctgggtca gacgatgagc ttcttcaatt ggcacgtcag 841 ttatacgaat tattagtacc ccaggcgatc ggtgcgaaag gcgacgctca gcagatcgcg 901 cgtaaattcc tgtccccgct ggctgataaa gatgctgtcg gtggcctggg aattgccaaa 961 gccgggaata aaccgcgttg ggtccgtatg cgcgaggctg gagagccggg atgggaagaa 1021 gagaaggcaa aagcggaagc ccgtaaatcc acggaccgta ctgcagacgt cttgcgcgcc 1081 ttagccgatt ttgggcttaa accgttgatg cgcgtctaca ccgactcaga tatgtcgtct 1141 gttcaatgga agcccctgcg taaagggcag gccgtacgta cctgggatcg tgatatgttt 1201 cagcaggcga tcgagcgtat gatgtcctgg gaatcctgga accaacgcgt aggcgaagca 1261 tacgcaaaat tggtcgaaca aaagtcgcgc ttcgagcaga agaactttgt cggacaggaa 1321 catctggttc aattggtcaa ccagctgcaa caggacatga aggaagcatc tcacggtctg 1381 gaatcgaaag agcaaactgc acactatctt actggtcgtg cgttgcgtgg tagtgataaa 1441 gtgtttgaga agtgggagaa gcttgatccc gatgcgcctt ttgatcttta cgacaccgag 1501 attaagaacg ttcaacgtcg caatacacgt cgctttggtt cgcatgactt attcgccaag 1561 ttggcagaac cgaaatacca agccctgtgg cgtgaagacg cgagctttct tacgcgttat 1621 gcagtgtaca atagtattgt ccgcaaactg aaccacgcca aaatgtttgc aacctttacg 1681 cttccagacg ccacagctca ccctatttgg acccgctttg ataaattggg gggtaatctt 1741 caccaatata cctttctgtt caacgagttc ggtgagggtc gccatgctat ccgcttccaa 1801 aagttactga cggtggaaga cggcgtggca aaggaagttg atgacgtgac tgtcccgatc 1861 agcatgtcag cccagttaga cgacctgctg ccgcgcgatc cgcatgaatt ggtcgcgctt 1921 tatttccaag attatggcgc ggaacaacac ttagcgggtg aattcggtgg ggcaaaaatc 1981 caatatcgtc gcgaccagct taaccacctg cacgctcgcc gcggtgcccg cgatgtttat 2041 ctgaaccttt cggtacgtgt gcagagtcaa tcagaggcac gcggcgaacg tcgtcctcca 2101 tatgctgcag tctttcgctt ggtaggggat aatcaccgcg catttgtgca tttcgataag 2161 ttatctgact accttgcaga acatccggat gatggtaaat tgggctcgga gggtctttta 2221 tcaggcttac gcgtaatgag tgtagacctg ggcctgcgta cttcagccag catctctgta 2281 tttcgcgtcg cacgtaaaga cgagttaaaa ccgaattccg agggtcgcgt tcctttctgc 2341 ttccctattg aagggaacga gaacctggta gcggttcacg aacgctccca attgttgaag 2401 ctgcccggag agacagaatc gaaggatctg cgtgctatcc gtgaggagcg ccagcgtacg 2461 ttgcgccaat tacgtacaca gcttgcatat ttgcgtcttt tagtacgctg tggttcagag 2521 gatgtgggtc gccgtgaacg ttcctgggca aagctgatcg aacaaccgat ggatgctaat 2581 cagatgaccc ccgactggcg cgaagctttc gaggacgagc tgcagaaact taagtcattg 2641 tatggtatct gtggagatcg tgaatggact gaggcagtct acgaatctgt gcgccgcgta 2701 tggcgccata tgggaaagca ggtacgcgac tggcgtaagg atgttcgttc cggcgaacgc 2761 cctaagattc gcggctatca gaaagacgtt gttggtggca attcgattga acagattgag 2821 tatcttgaac gtcagtacaa atttctgaag tcttggtcat tctttggaaa agtatcaggt 2881 caggtaattc gtgccgagaa ggggtcccgc ttcgctatca ccttacgcga acatatcgat 2941 catgcgaaag aagaccgcct taaaaaattg gctgatcgta tcatcatgga ggcattagga 3001 tatgtgtatg cactggacga cgaacgtggc aaagggaaat gggttgccaa gtatccgccg 3061 tgtcaattga ttttactgga agaattgtcg gagtaccagt ttaacaacga ccgtcccccc 3121 tcagagaata accagcttat gcaatggtcg catcgtggag tgttccaaga actgttaaac 3181 caagcacaag ttcatgacct tcttgtaggt actatgtatg cggcattttc cagtcgcttt 3241 gacgcacgta cgggggctcc ggggatccgt tgtcgtcgcg tacctgcacg ttgcgcacgc 3301 gaacagaatc cggagccttt tccttggtgg ttgaataagt tcgttgcgga gcataagttg 3361 gacggctgcc ctttgcgcgc tgatgacctg atccccacag gcgaaggtga gttcttcgtt 3421 agtccatttt cagctgagga gggtgacttt caccagatcc atgccgacct taacgccgcc 3481 caaaatttgc aacgtcgtct ttggtcagac tttgatatct cgcaaattcg tctgcgctgt 3541 gattgggggg aggttgatgg cgagccagtt ttaattccgc gcacaactgg caagcgtacc 3601 gctgacagct acggcaacaa ggttttttat accaagaccg gtgtaacgta ttacgaacgt 3661 gagcgcggta aaaaacgtcg caaagttttt gctcaggaag aactgagtga agaagaggct 3721 gaacttttgg tggaagccga tgaggcccgt gagaaatcag tagttttgat gcgtgatccg 3781 tcaggaatca ttaatcgtgg agactggacg cgtcaaaaag aattttggag tatggtcaac 3841 caacgcatcg agggttacct tgttaagcag atccgctcgc gcgtccgtct gcaggaaagt 3901 gcttgtgaga acactgggga catctgagcg gccgcactcg aggcccgaaa ggaagctgag 3961 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc 4021 ttgaggggtt ttttgctgaa aggaggaact atatccggat atcccgcaag aggcccggca 4081 gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga 4141 tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata 4201 aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga 4261 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag 4321 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 4381 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 4441 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 4501 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 4561 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 4621 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 4681 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat 4741 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 4801 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 4861 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 4921 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 4981 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa 5041 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 5101 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 5161 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 5221 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 5281 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 5341 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 5401 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 5461 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 5521 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 5581 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta 5641 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 5701 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 5761 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 5821 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 5881 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 5941 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 6001 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 6061 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 6121 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 6181 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg 6241 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt 6301 tcacaccgca atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt 6361 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc 6421 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac 6481 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca 6541 gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc 6601 cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc 6661 catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct 6721 gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga 6781 tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg 6841 gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg 6901 tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg 6961 cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt 7021 tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga 7081 ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag 7141 cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct 7201 gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt 7261 tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg 7321 ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg 7381 cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag 7441 gcggcataaa tcgccgtgac gatcagcggt ccaatgatcg aagttaggct ggtaagagcc 7501 gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc 7561 tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc 7621 cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg 7681 ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg 7741 agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga 7801 aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg 7861 ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag 7921 ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc 7981 taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 8041 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag 8101 ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc 8161 ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt 8221 gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac 8281 cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc 8341 catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat 8401 ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat 8461 ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact 8521 taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc 8581 cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac 8641 atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc 8701 atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc 8761 cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag 8821 ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact 8881 ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt 8941 gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac 9001 gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc 9061 gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg 9121 ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct 9181 ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca 9241 ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg 9301 ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat 9361 cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga 9421 tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat //
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇:BB3cH_pGAP_23*_pLAT1_Cas9
- 下一篇:没有了